Pengembangan Rosella Ungu (Hibiscus sabdariffa) sebagai Minuman Isotonik Berpotensi Antioksidan dan Mampu Meningkatan Kebugaran Tubuh
Setyaningrum Ariviani(1*), Gusti Fauza(2), Cristiva Pawestri(3)
(1) Program Studi Ilmu dan Teknologi Pangan, Fakultas Pertanian, Universitas Negeri Sebelas Maret, Jl. Ir. Sutami No 36 A, Kentingan, Surakarta 57126
(2) Program Studi Ilmu dan Teknologi Pangan, Fakultas Pertanian, Universitas Negeri Sebelas Maret, Jl. Ir. Sutami No 36 A, Kentingan, Surakarta 57126
(3) Program Studi Ilmu dan Teknologi Pangan, Fakultas Pertanian, Universitas Negeri Sebelas Maret, Jl. Ir. Sutami No 36 A, Kentingan, Surakarta 57126
(*) Corresponding Author
Abstract
Exercise induces more body fluid loss and free radicals formation. Therefore, the body requires an intake of isotonic drink that rich in antioxidant. Plasma antioxidant has an effect on the human physical fitness. Having high level of anthocyanin and vitamin C, dark red roselle (Hibiscus sabdariffa L) has antioxidant capacity. However, the application of dark red roselle extract as an isotonic drink has not yet been reported. This research was aimed to develop red dark roselle as an isotonic drink with high level of antioxidant and ability to increase human physical fitness in exercise. This study was conducted in two stages. The first stage was extraction of dark red roselle with different solvent. The extract with highest antiradical activity was used for isotonic drink formulation. In the second stage, the antioxidant capacity and its effectiveness on improving physical fitness in exercise were evaluated on the isotonic drink with the highest level of consumer preference. The result showed that extraction technique using distilled water gave an extract with the highest antiradical activity. The isotonic drink containing 50% dark red roselle extract showed the highest consumer preference level. The drink showed to have potentially antiradical and capability for increasing physical fitness in exercise with the score of PFI (physical fitness index) reached 52.3 indicating a medium level of physical fitness.
ABSTRAK
Olahraga menginduksi kehilangan cairan tubuh dan pembentukan radikal bebas yang lebih banyak. Oleh karena itu, tubuh membutuhkan asupan minuman isotonik yang kaya antioksidan. Antioksidan plasma berpengaruh pada kebugaran tubuh manusia. Rosella ungu (Hibiscus sabdariffa L) memiliki kapasitas sebagai antioksidan karena memiliki kadar antosianin dan vitamin C yang tinggi. Aplikasi ekstrak rosella ungu sebagai minuman isotonik belum pernah dilakukan sebelumnya. Penelitian ini bertujuan untuk mengembangkan rosella ungu sebagai minuman isotonik yang berpotensi antioksidan dan mampu meningkatkan kebugaran tubuh pasca stress fisik. Penelitian ini dilakukan dalam beberapa tahap. Ekstraksi rosella ungu menggunakan pelarut yang berbeda dilakukan pada tahap pertama. Ekstrak dengan aktivitas antiradikal tertinggi digunakan untuk formulasi minuman isotonik. Pada tahap terakhir, minuman isotonik dengan tingkat kesukaan konsumen tertinggi dievaluasi kapasitas antioksidan dan efektivitasnya dalam peningkatan kebugaran tubuh pasca stress fisik. Hasil penelitian menunjukkan bahwa teknik ekstraksi menggunakan air sebagai pelarut menghasilkan ekstrak dengan aktivitas antiradikal tertinggi, yaitu mencapai ekuivalen 93,16 ± 3,94 mmol BHT/L. Minuman isotonik dengan penggunaan 50% ekstrak rosella ungu menunjukkan tingkat kesukaan konsumen tertinggi. Minuman isotonik rosella ungu terbukti berpotensi sebagai antiradikal dan mampu meningkatkan kebugaran tubuh pasca stress fisik dengan skor IKJ (indeks kesegaran jasmani) mencapai 52,3 yang mengindikasikan tingkat kebugaran sedang.
Keywords
Full Text:
PDFReferences
Ali, B.H., Wabel, N.A. dan Blunden, G. (2005). Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L.: a review. Phytotherapy Research 19: 369–375. doi: 10.1002/ptr.162.
Ariviani, S. dan Parnanto, N.H.R. (2013). Kapasitas antioksidan buah salak (Salacca edulis Reinw) kultivar Pondoh, Nglumut dan Bali serta korelasinya dengan kadar fenolik total dan vitamin C. Agritech 33(3): 324–333.
Borowski, L. (1988). Sweating: Student find exercise and dehydration to be hot topics in chemistry. The Science Teacher Journal 65(7): 20–25.
Borrás-Linares, I., Fernández-Arroyo, S., Arráez-Roman, D., Palmeros-Suárez, P.A., Val-Díaz, R.D, Andrade-Gonzáles, I., Fernández-Gutiérrez, A., Gómez-Leyva, J. F. dan Segura-Carretero, A. (2015). Characterization of phenolic compounds, anthocyanidin, antioxidant and antimicrobial activity of 25 varieties of Mexican Roselle (Hibiscus sabdariffa L.). Industrial Crops and Products 69: 385–394. doi: 10.1016/j.indcrop.2015.02.053.
Cao, S., Liu, L., Lu, Q., Xu, Y., Pan, S. dan Wang, K. (2009) Integrated effects of ascorbic acid, flavonoids and sugarson thermal degradation of anthocyanins in blood orange juice. European Food Research Technology 228: 975–983. doi: 10.1007/s00217-009-1015-2.
Cases, N., Aguilo, A., Tauler, P., Sureda, A., Lompart, I., Pons, A. dan Tur, J.A. (2005). Differential responses of plasma and immune cell’s vitamin E levels to physical activity antioxidant vitamin supplementation. European Journal of Clinical Nutrition 59(6): 781–788. doi: 10.1038/sj.ejcn.1602143.
Castaneda-Ovando, A. Pacheco-Hernandez, M. L., Paez-Hernandez, M.E, Rodriguez, J.A. dan Galan-Vidal, C.A. (2009). Chemical studies of anthocyanins: A review. Food Chemistry 113: 859–871. doi: 10.1016/j.foodchem.2008.09.001.
Cavalcanti, R.N., Santos, D.T. dan Meireles, M.A.A. (2011). Non-thermal stabilization mechanisms of anthocyanins in model and food systems-An overview. Food Research International 44: 499–509. doi: 10.1016/j.foodres.2010.12.007.
Chawla, K., Mishra, R., Sachdeva, V. dan Beenu. (2007). Correlation of antioxidant and fitness levels in undergraduate medical students. Indian Journal of Physiology and Pharmacology 51(3): 293–295.
Christian, K.R., Nair, M.G. dan Jackson, J.C. (2006). Antioxidant and cyclooxygenase inhibitory activity of sorrel (Hibiscus sabdariffa). Journal of Food Composition and Analysis 19(8): 778–783. doi: 10.1016/j.jfca.2006.04.004.
Clarkson, P.M. dan Thompson, H.S. (2000). Antioxidants: what role do they play in physical activity and health? The American Journal of Clinical Nutrition 72: 637S–646S.
Costill, D.L. (1988). Carbohydrate for exercise: dietary demands for optimal performance. International Journal of Sports Medicine 9(1): 1–18. doi: 10.1055/s-2007-1024971.
Christian, K.R. dan Jackson, J.C. (2009). Changes in total phenolic and monomeric anthocyanin composition and antioxidant activity of three varieties of sorrel (Hibiscus sabdariffa) during maturity. Journal of Food Composition and Analysis 22(7): 663–667. doi: 10.1016/j.jfca.2009.05.007.
Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I. dan Heinrich, M. (2014). Hibiscus sabdariffa L.–A phytochemical and pharmacological review. Food Chemistry 165: 424–443. doi: 10.1016/j.foodchem.2014.05.002.
Davis, J.M., Lamb, D.R., Pate, R.R., Slentz, C.A., Burgess, W.A. dan Bartoli, W.P. (1988). Carbohydrate-electrolyte drinks: effects on endurance cycling in the heat. The American Journal of Clinical Nutrition 48: 1023–1030.
De Rosso, V.V. dan Mercadante, A.Z. (2007a). The high ascorbic acid content is the main cause of the low stability of anthocyanin extracts from Acerola. Food Chemistry 103: 935–943. doi: 10.1016/j.foodchem.2006.09.047.
De Rosso, V.V. dan Mercadante, A.Z. (2007b). Evaluation of colour and stability of anthocyanins fromtropical fruits in an isotonic soft drink system. Innovative Food Science and Emerging Technologies 8: 347– 352. doi: 10.1016/j.ifset.2007.03.008.
DepKes RI. (1994). Pedoman Pengukuran Kesegaran Jasmani. Direktorat Bina Kesehatan Puskesmas. Jakarta.
Dhanutirto, H. (1970). Kesanggupan Badan. Tesis. Fakultas Kedokteran. Universitas Indonesia, Jakarta.
Dyrby, M., Westergaard, N. dan Stapelfeldt, H. (2001). Light and heat sensitivity of red cabbage extract in soft drink model systems. Food Chemistry 72: 431–437. doi: 10.1016/S0308-8146(00)00251-X.
Federal Register of Legislative Instruments (2009). Standard 2.6.2 Non Alcoholic Beverages and Brewed Soft Drink. Federal Register of Legislative Instruments, New Zeland.
Finaud, J., Gerard, L. dan Edith, F. (2006). Oxidative stress: relationship with exercise and training. Journal of Sports Medicine 36(4): 327– 358.
Francis, F.J. (1989). Colorants. Eagan Press, Minnesota. USA.
Gabrielska, J. dan Oszmianski, J. (2005). Antioxidant activity of anthocyanin glycoside derivatives evaluated by the inhibition of liposome oxidation. Zeitschrift für Naturforsch 60c: 399–407.
Garcia-Viguera, C. dan Bridle, P. (1999). Influence of structure on colour stability of anthocyanins and flavylium salts with ascorbic acid. Food Chemistry 64: 21–26. doi: 10.1016/S0308-8146(98)00107-1.
Giusti, M.M. dan Worsltad, R.E. (2001). Characterization and measurement of anthocyanins by UV-visible Spectroscopy. Dalam: Wrolstad, R.E., Acree, T.E., Decker, E.A., Penner, M.H., Reid, D.S., Schwartz, S.J., Shoemaker, C.F., Smith, D.M. dan Sporns, P. (ed). Current Protocol in Food Analytical Chemistry. Wiley, New York.
Harborne, J.B. dan Williams, C.A. (1987). Anthocyanins and other flavonoids. Natural Product Reports 18: 310–333. doi: 10.1039/NP9951200639.
Hernández, Y., Lobo, M.G. dan González, M. (2006). Determination of vitamin C in tropical fruits: a comparative evaluation of methods. Food Chemistry 96: 654–664. doi: 10.1016/j.foodchem.2005.04.012.
Ihara, H., Shino, Y., Morita, Y., Kawaguci, E., Hashizume, N. dan Yoshida, M. (2001). Is skeletal muscle damaged by the oxidative stress following anaerobic exercise? Journal of Clinical Laboratory Analysis 15: 239–243.
Jakeman, P. dan Maxwell, S. (1993). Effect of antioxidant vitamin supplementation on muscle function after eccentric exercise. European Journal of Applied Physiology 67: 426–430.
Kahkonen, M.P. dan Heinonen, M. (2003). Antioxidant activity of anthocyanins and their aglycons. Journal of Agriultural and Food Chemistry 51(3): 628–633. doi: 10.1021/jf025551i.
Kong, J-M., Chia, L-S., Goh, N-K., Chia, T-F. dan Brouillard, R. (2003). Analysis and biological activities of anthocyanins. Phytochemistry 64: 923–933. doi: 10.1016/S0031-9422(03)00438-2.
Krings, U. dan Berger, R.G. (2001). Antioxidant activity of some roasted foods. Food Chemistry 72: 223–229. doi: 10.1016/S0308-8146(00)00226-0.
Lapornik, B., Prosek, M. dan Wondra, A.G. (2005). Comparison of extracts prepared from plant by-products using different solvents and extraction time. Journal of Food Engineering 71: 214–222. doi: 10.1016/j.jfoodeng.2004.10.036.
Lecarpentier, Y. (2007). Physiological role of free radicals in skeletal muscles. Journal of Applied Physiology 103: 1917–1918. doi: 10.1152/japplphysiol.01047.2007.
Malien-Aubert, C., Dangles, O. dan Amiot, M.J. (2001). Color stability of commercial anthocyanin-based extracts in relation to the phenolic composition. Protective effects by intra-and intermolecular copigmentation. Journal Agriulture and Food Chemistry 49(1): 170–176.
Maud, P.J. dan Foster, C. (1995). Physiological Assessment of Human Fitness. Human Kinetics Publisher, Compaign, Illinois, USA.
Meilgaard, M., Civille, G.V. dan Carr, B.T. (1999). Sensory Evaluation Techniques. Third edition. CRC Press, Boca Raton.
Morillas-Ruiz, J., Garcia, J.A.V., Lopez, F.J. dan Vidal-Guevara, Z.P. (2006). Effect of polyphenolic antioxidants on exercise-induced oxidative stress. Clinical Nutrition 25: 444–453. doi:10.1016/j.clnu.2005.11.007.
Murray, R.S. dan Undermann, B.E. (2003). Fluid replacement: a historical perspective and critical review. International Sports Journal 7(2): 58–73.
Narwidina, P. (2010). Pengembangan Minuman Isotonik Antosianin Beras Hitam (Oryza sativa I. indica) dan Efeknya terhadap Kebugaran dan Aktivitas Antioksidan pada Manusia Pasca Stres Fisik: A Case Control Study. Thesis. Fakultas Teknologi Pertanian. Universitas Gadjah Mada, Yogyakarta.
Nio, O.K. (1992). Daftar Analisis Bahan Makanan. Fakultas Kedokteran, Universitas Indonesia Press. Jakarta.
Pacheco-palencia, L.A., Hawken, P. dan Talcot, S.T. (2007). Juice matrix composition and ascorbic acid fortification effects on the phytochemical, antioxidant and pigment stability of acai (Euterpe oleracea Mart.). Food Chemistry 105: 28–35. doi: 10.1016/j.foodchem.2007.03.027.
Padayatty, S.J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J.H., Chen, S., Corpe, C., Dutta, A., Dutta, S.K. dan Levine, M. (2003). Vitamin C as an antioxidant: evaluation of its role in disease prevention. Journal of the American College of Nutrition 22(1): 18–35.
Pérez-Ramírez, I. F., Castaño-Tostado, A., Ramírez-de León, J.A., Rocha-Guzmá, N.E. dan Reynoso-Camacho, R. (2015). Effect of stevia and citric acid on the stability of phenolic compounds and in vitro antioxidant and antidiabetic capacity of a roselle (Hibiscus sabdariffa L.) beverage. Food Chemistry 172: 885–892. doi: 10.1016/j.foodchem.2014.09.126.
Pokorny, J., Yanishlieva, N. dan Gordon, M. (2001). Antioxidants in Food. CRC Press Cambridge. England.
Suharto (1997). Kebugaran Jasmani dan Peranannya, Informasi, Kesehatan, dan Olahraga. Pusat Komunikasi Pemuda. Jakarta.
Suntornsuk, L., Gritsanapun, W., Nilkamhank, S. dan Paochom, A. (2002). Quantitation of vitamin C content in herbal juice using direct titration. Journal of Pharmaceutical and Biomedical Analysis 28(5): 849–855. doi: 10.1016/S0731-7085(01)00661-6.
Tsai, P-J. dan Huang, H-P. (2002). Effect of polymerization on the antioxidant capacity of anthocyanins in roselle (Hibiscus sabdariffa L.) extract. Food Research International 37: 313–318. doi: 10.1016/j.foodres.2003.12.007.
Turker, N. dan Erdogdu, F. (2006).Effects of pH and temperature of extraction medium on effective diffusion coefficient of anthocyanin pigmentsof black carrot (Daucus carota var. L.). Journal of Food Engineering 76: 579–583. doi: 10.1016/j.jfoodeng.2005.06.005.
Wahjoedi (1999). Landasan Evaluasi Pendidikan Jasmani Edisi I. PT. Raja Grafindo Persada. Jakarta.
Walton, M.C. (2006). Berry Fruit Anthocyanin and Human Nutrition-Bioavailability and Antioxidant Effects. Thesis. Nutritional Science. Massey University, Palmerson North, New Zeland.
Wang, L-S. dan Stoner, G.D. (2008). Anthocyanins and their role in cancer prevention. Cancer Letters 269(2): 281–290. doi: 10.1016/j.canlet.2008.05.020.
Wong, P-K, Yusof, S., Ghazali, H.M. dan Man, Y.B.C. (2002). Physico-chemical Characteristics of roselle (HibiscussabdariffaL.). Nutrition and Food Science 32(2): 68–73. doi: 10.1108/0034665021041699.
DOI: https://doi.org/10.22146/agritech.12739
Article Metrics
Abstract views : 7043 | views : 9647Refbacks
- There are currently no refbacks.
Copyright (c) 2017 Setyaningrum Ariviani, Gusti Fauza, Cristiva Pawestri
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
agriTECH has been Indexed by:
agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.