Aktivitas Antioksidan Bubuk Sargassum hystrix Selama Penyimpanan pada Suhu Berbeda

https://doi.org/10.22146/agritech.18134

Pingkan Mayestika Afgatiani(1), Amir Husni(2*), Siti Ari Budhiyanti(3)

(1) Departemen Perikanan, Fakultas Pertanian, Universitas Gadjah Mada. Jalan Flora Gedung A4 Bulaksumur Yogyakarta 55281
(2) Departemen Perikanan, Fakultas Pertanian, Universitas Gadjah Mada. Jalan Flora Gedung A4 Bulaksumur Yogyakarta 55281
(3) Departemen Perikanan, Fakultas Pertanian, Universitas Gadjah Mada. Jalan Flora Gedung A4 Bulaksumur Yogyakarta 55281
(*) Corresponding Author

Abstract


The aim of this study was to determine the effect of storage temperature on the antioxidant activity of Sargassum hystrix. Samples were collected from Sepanjang Beach Gunung Kidul, Yogyakarta, and dried, pulverized, and then stored for two months at room temperature (17-33 °C), refrigerator (4 °C) and freezer (-18°C). Furthermore, the samples were tested every two weeks to determine their water content, total phenol, DPPH, and FIC. Also, Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed for two months before and after storage. The results showed that the water content increased after treatments, along with storage time which ranges from 8.55 to 14.27 %. Total phenol before storage was 175.73 mg GAE/g extract, and freezing has the highest total phenol levels after storage which was 22.07 mg GAE/g extract. Also, DPPH testing showed S. hystrix has an
IC50 value of 0.45 mg/mL, and refrigeration has the lowest IC50 value of 3.18 mg/mL. FIC test showed IC50 value of 1.44 mg/mL, and freezing has the lowest FIC IC50 value of 15.36 mg/mL. In addition, GC-MS sample analysis before storage detected 8 compounds with antioxidant activity of 86.96 %. After two months, they were detected at room temperature, refrigerator, and freezing which are 4, 3, and 8 compounds respectively.

Keywords


Antioxidant; DPPH; FIC; storage temperature; Sargassum hystrix

Full Text:

PDF


References

Asgar, A., & Rahayu, S.T. (2014). Pengaruh suhu penyimpanan dan waktu pengkondisian untuk mempertahankan kualitas kentang kultivar Margahayu. Berita Biologi, 13: 283–293. http://dx.doi.org/10.14203/beritabiologi.v13i3.672

BSN (Badan Standarisasi Nasional). (2006). Cara Uji Kimia- Bagian 2: Penentuan Kadar Air pada Produk Perikanan. SNI-01-2354.2-2006. Standar Nasional Indonesia (SNI).

Budhiyanti, S.A., Raharjo, S., Marseno, D.W., dan Lelana, I.Y.B. (2011). Free radical scavenging, metal chelating, and singlet oxygen quenching activity of fractionated brown seaweed Sargassum hystrix extract. Journal of Biological Sciences, 3: 1–11. http://dx.doi.org/10.3923/jbs.2011.288.298

Cahyaningrum, K., Husni, A., & Budhiyanti, S.A. (2016). Aktivitas antioksidan ekstrak rumput laut cokelat (Sargassum polycystum). Agritech, 36: 137-144. https://doi.org/10.22146/agritech.12857

Cao, S.F., Zheng, Y.H., Yang, Z.F., Li, N., Ma, S.J., Tang, S.S., & Zhang, J.H. (2007). Effects of storage temperature on antioxidant composition and anti-oxidant activity of loquat fruit. Acta Horticulturae, 750: 471–47. DOI: 10.17660/ActaHortic.2007.750.75

Cuong, D.X., Boi, V.N., Van, T.R.T., & Hau, L.N. (2015). Effect of storage time on phlorotannin content and antioxidant activity of six Sargassum species from Nathrang Bay, Vietnam. Journal of Application and Phycology, 2: 567–572. https://doi.org/10.1007/s10811-015-0600-y

Ebrahimzadeh, M.A., Pourmorad, F., & Bekhradnia, A.R. (2008). Iron chelating activity, phenol and flavonoid content of some medicinal plants. African Journal of Biotechnology, 7: 3188–3192. DOI: 10.3923/pjbs.2009.934.938

Gomez, K.A. & Gomez A.A. (1984). Statistical Procedures for Agricultural Research. John Willey and Sons. Canada.

Hossain, M.D., Sarwar, M.S., Dewan, S.M.R., Hossain, M.S., Shahid-Ud-Daula, A.F.M. & Islam, M.S. (2014). Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots. Avicenna Journal of Phytomedicine, 4: 97–102.

Husni, A., Putra, D.R., & Lelana, I.Y.B. (2014). Aktivitas antioksidan Padina sp. pada berbagai suhu dan lama pengeringan. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 9: 165–173. http://dx.doi.org/10.15578/jpbkp.v9i2.109

Juniarka, I.G.A., Lukitaningsih, E., & Noegrohati, S. (2011). Analisis aktivitas antioksidan dan kandungan antosianin total ekstrak dan liposom kelopak bunga Rosella (Hibiscus sabdariffa L.). Majalah Obat Tradisional, 16: 115–123.

Kang, C., Jin, Y.B., Lee, H., Cha, M., Sohn, E., Moon, J., Park, C., Chun, S., Jung, E.S., Hong, J.S., Kim, S.B., Kim, J.S., & Kim, E. (2010). Brown alga Ecklonia cava attenuates type 1 diabetes by activating AMPK and akt signaling pathways. Food and Chemical Toxicology, 48: 509–516. https://doi.org/10.1016/j.fct.2009.11.004

Khaira, K. (2010). Menangkal radikal bebas dengan antioksidan. Jurnal Saintek, 2: 183-187.

Kim, S., Woo, S., Yun, H., Yum, E., Choi, E., Do, J.R., Jo, J.H., Kim, D., Lee, S., & Lee, T.K. (2005). Total phenolic contents and biological activities of Korean seaweed extracts. Food Science and Biotechnology, 14: 798-802

Lailiyah, A., Adi, T.K., Hakim, A., &Yusnawan, E. (2014). Kapasitas antioksidan dan kandungan total senyawa fenolik ekstrak kasar alga coklat Sargassum cristaefolium dari Pantai Sumenep Madura. Alchemy, 3: 18–30. DOI: 10.18860/al.v0i0.2902

Maulida, R., & Guntarti, A. (2015). Pengaruh ukuran partikel beras hitam (Oryza sativa L.) terhadap rendemen ekstrak dan kandungan total antosianin. Pharmaciana, 5: 9–6. http://dx.doi.org/10.12928/pharmaciana.v5i1.2281

Molyneux, P. (2004). The use of the stable free radical Diphenyl Picryl Hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26: 211-219.

Monica, A., Madrau, Piscopo, A., Sanguetti, A.M., Caro, A.D., Poina, M., Romeo, F.V., & Piga, A. (2009). Effect of drying temperature on polyphenolic content and antioxidant activity of Apricots. Europe Food Reseacrh Technology, 228: 441–448. DOI: 10.1007/s00217-008-0951-6

Nurhayati, Siadi, K., & Harjono. (2012). Pengaruh konsentrasi Natrium Benzoat dan lama penyimpanan pada kadar fenolat total pasta tomat. Indonesian Journal of Chemical Science, 1: 159–163.

Pustiari, P.A., Leliqia, N.P.E., & Wijayanti, N.P.A.D. (2014). Penentuan rendemen antosianin total ekstrak kulit buah Manggis (Garciania mangostana, L) dengan pengeringan oven. Jurnal Farmasi Udayana, 3: 9-12.

Reblova, Z. (2012). Effect of temperature on the antioxidant activity of phenolic acids. Czech Journal of Food Science, 30: 171-177. DOI: 10.17221/57/2011-CJFS

Sarojini, Y.B., Sujatha, & Lakshinarayana, K. (2013). Total phenol content and antioxidant activities of ethanolic extracts of two marine brown macroalgae. International Journal of Current Science, 8: 4 –49.

Sen, S., Raja, C., Sridhar, C., & Reddy, Y.S.R. (2010). Free radicals, antioxidants, diseases and phytomedicines: Current status and future prospect. International Journal of Pharmaceutical Sciences, 3: 91-100.

Septiana, A.T., & Asnani, A. (2013). Aktivitas antioksidan ekstrak rumput laut Sargassum duplicatum. Jurnal Teknologi Pertanian, 14: 79-86. https://jtp.ub.ac.id/index.php/jtp/article/view/396/757

Zavala, J.F.A., Wang, S.Y., Wang, C.Y., &Agular, G.A.G. (2004). Effect of storage temperatures on antioxidant capacity and aroma compounds in strawberry fruit. Lebensm.-Wiss. U.-Technology, 37: 687–695. DOI: 10.1016/j.lwt.2004.03.002

Zubia, M., Fabre, M.S., Kerjean, V., Lann, K.L., Stiger-Pouvreau, V., Fauchon, M., & Deslandes, E. (2009). Antioxidant and antitumoural activities of some phaeophyta from Brittany coasts. Food Chemistry, 116: 693–701. https://doi.org/10.1016/j.foodchem.2009.03.025



DOI: https://doi.org/10.22146/agritech.18134

Article Metrics

Abstract views : 3574 | views : 6456

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2020 Pingkan Mayestika Afgatiani, Amir Husni, Siti Ari Budhiyanti

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

agriTECH has been Indexed by:

Emerging Source Citation Index Directory of Open Access Journals CABI.org Dimensions Google Scholar Sinta Garuda

agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.

website statisticsView My Stats