* Corresponding Author
Sifat-Sifat Fisikokimia Pati Ubi Kayu Terfermentasi Khamir Indigenus Tapai
https://doi.org/10.22146/agritech.26323 Heru Widyatmoko(1), Achmad Subagio(2), Nurhayati Nurhayati(3*)
(1) Program Studi Magister Bioteknologi, Universitas Jember,Jl. Kalimantan No.37 PS BIOTEKNOLOGI UNEJ, Sumbersari, Jember 68121
(2) Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember, Jl. Kalimantan No.37 FTP UNEJ, Sumbersari, Jember 68121
(3) Center for Development of Advanced Science andTechnology, Universitas Jember, Jl. Kalimantan No.37 CDAST UNEJ, Sumbersari, Jember 68121
(*) Corresponding Author
Abstract
Tapai is a cassava food product fermented by yeast starter (“Ragi”). Among the amylolitic yeasts that had been isolated from the tapai were Candida guilliermondii, C. tropicalis, Trichosporon mucoides, and Saccharomycopsis fibuligera. The aim of this research was to characterize the physicochemical properties of fermented cassava starch by amylolytic yeasts of indigenus tapai i.e. C. guilliermondii, C. tropicalis, T. mucoides, and S. fibuligera. The process of fermentation of cassava starch was done by inoculating each pure isolate by 5% v / v (105 CFU / ml) into cassava starch suspension 50% b/v (100g starch into 200ml sterile distilled water). The incubation was carried out at 28 °C for 24 h, 48 h, and 72 h. The fermented cassava starch was dried at 50 °C for 24 h. Characterization of physicochemical properties of cassava starch included chemical properties (amylose content), paste properties (RVA), thermal properties (DSC), physical properties (SEM granule macrostructure) and functional groups (FT-IR) of unfermented (native) or fermented cassava starch. The results showed that during the fermentation process, the yeast was able to grow up to 7 log10 CFU/ml and the acidity decreased to pH 3,9. The amylose content of fermented cassava starch decreased to 11,22% from native starch amylose content (15,85%). The paste properties of fermented cassava starch increased at the value of trough viscosity, breakdown, final viscosity, and swelling power. The thermal properties of fermented cassava starch increased to 64,7-67 °C for the initial temperature value of gelatinization (To), 69,1-71,2 °C for gelatinization peak temperature (Tp), 73,9-75 °C for the final temperature of gelatinization (Tc), but decreased gelatinization enthalpy in the range of ΔH 4,84-6,38 J/g. The macrostructure of the longest fermented starch granules (72 hours)occurred liberation into irregular granular shapes. The profile of fermented cassava starch functional groups had a similarity with native cassava starch that had the highest absorption spectra at 3291,2 cm-1 peak (vibration of the O-H bonding group) and 2920 cm-1 peak (C-H group vibration).
ABSTRAK
Tapai merupakan produk pangan ubi kayu yang terfermentasi oleh starter khamir (ragi). Di antara khamir amilolitik yang sudah diisolasi dari tapai yaitu Candida guilliermondii, C. tropicalis, Trichosporon mucoides, dan Saccharomycopsis fibuligera. Tujuan penelitian ini adalah melakukan karakterisasi sifat-sifat fisikokimia pati ubi kayu yang difermentasi oleh khamir amilolitik indigenus tapai yaitu C. guilliermondii, C. tropicalis, T. mucoides, dan S. fibuligera. Proses fermentasi pati ubi kayu dilakukan dengan menginokulasikan masing-masing isolat murni sebanyak 5% v/v (105 CFU/ml) ke dalam suspensi pati ubi kayu 50% b/v (100g pati ke dalam 200ml akuades steril). Selanjutnya dilakukan inkubasi pada suhu 28 °C selama 24, 48, dan 72 jam. Pati ubi kayu terfermentasi dikeringkan pada suhu 50 oC selama 24 jam. Karakterisasi sifat-sifat fisikokimia pati ubi kayu meliputi sifat kimia (kadar amilosa), sifat pasta (RVA), sifat termal (DSC), sifat fisik (makrostruktur granula SEM) dan gugus fungsi (FT-IR) pati ubi kayu alami maupun pati ubi kayu terfermentasi. Hasil penelitian menunjukkan bahwa selama proses fermentasi, khamir mampu tumbuh hingga mencapai 7 log10 CFU/ml dan derajat keasaman mengalami penurunan mencapai pH 3,9. Kadar amilosa pati ubi kayu terfermentasi mengalami penurunan menjadi 11,22% dari kadar amilosa pati alami (15,85%). Sifat pasta pati ubi kayu terfermentasi khamir mengalami peningkatan pada nilai viskositas trough, breakdown, viskositas akhir, dan daya kembang. Sifat termal pati ubi kayu terfermentasi menglamai peningkatan menjadi 64,7-67 °C untuk nilai suhu awal gelatinisasi (To), 69,1-71,2 °C untuk suhu puncak gelatinisasi (Tp), 73,9-75 °C untuk suhu akhir gelatinisasi (Tc), tetapi mengalami penurunan entalpi gelatinisasi pada kisaran ∆H 4,84-6,38 J/g. Makrostruktur granula pati yang terfermentasi paling lama (72 jam) mengalami liberasi dengan bentuk granula yang tidak beraturan. Profil gugus fungsi pati ubi kayu terfermentasi oleh khamir memiliki kemiripan dengan pati ubi kayu alami yaitu memiliki spektra penyerapan tertinggi pada puncak 3291,2 cm-1 (getaran gugus ikatan O-H) dan puncak 2920 cm-1 (getaran gugus C-H).
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Alonso-Gomez, L., Nino-Lopez, A. M., Romero-Garzon, A. M., Pineda-Gomez, P., del Real-Lopez, A., dan Rodrigues-Garcia, M. E. (2016). Physicochemical transformation of cassava starch during fermentation for production of sour starch in Columbia. Starch-Starke 68: 1139-1147.
AOAC (1995). Official methods of Analysis of The Association Analytical Chemists (AOAC). Dipublikasi oleh: TheAssociation of Official Analytical Chemist. Washington DC, USA.
Angeloni, M. M. J., Kurtz, D. J., Raguzzoni, J. C., Delgadillo, I., Maraschin, M., Soldi, V., Reginatto, V., dan Amante, E. R. (2009). Expansion properties of sour cassava starch (Polvilho Azedo): variable related to its practical application in bakery. Starch-Starke 61 (12): 716-726.
Armanto, R., dan Nurasih, A. S. (2008). Kajian konsentrasi bakteri asam laktat dan lama fermentasi pada pembuatan tepung pati singkong asam. Agritech 28 (3): 97-101.
Chen, B., Dang, L., Zhang, X., Fang, W., Hou, M., Liu, T., dan Wang, Z. (2017). Physicochemical properties and micro-structural characteristic in starch from kudzu root as affected by cross-linking. Food Chemistry 219 (1): 93-101.
Chinsamran, K., Piyachomkwan, K., dan Santisopasri, V. (2005). Effect of lactic acid fermentation on physico-chemical properties of starch derived from cassava, sweet potato and rice. Kasetsart J 39 (9): 76-2005.
Copeland, L., Blazek, J., Salman, H., dan Tang, M. C. (2009). Food hydrocolloids form and functionally of starch. Food hydrocolloids 23 (6): 1527-1534.
De, S. A. C. M., Azevedo, M. S., Ribeiro, D. H. B., Costa, A. C. O., dan Amante, E. R. (2014). Validation of HPLC and CE methods for determination of organic acids in sour cassava starch wastewater. Food Chemistry 172 (1): 725-730.
Dura, A., Blaszczak, W., dan Rosell, C. M. (2014). Functionality of porous starch obtained by amylase or amyglucosidase treatments. Carbohydrate polymers 101 (1): 837-845.
El-Sharoud, W. M., Belloch, C., Peris, D., dan Querol, A. (2009). Molecular identification of yeasts associated with traditional egyptian dairy products. Journal of Food Science 74 (7): 1-6.
Freire, A. L., Ramos, C. L., dan Schwan, R. F. (2015). Microbiological and chemical parameters during cassava based-substrate fermentation using potential starter cultures of lactic acid bacteria and yeast. Food Research International 76: 787-795.
Garcia, M. C., Franco, C. M. L., Junior, M. S. S., dan Caliari, M. (2016). Structural charateristics and gelatinization properties of sour cassava starch. Journal of Thermal Analysis and Calorimetry 123 (2): 919-926.
Gunawan, S., Widjaja, T., Zullaikah, S., Ernawati, L., Istianah, N., Aparamarta, H. W., dan Prasetyo, D. (2015). Effect of fermenting cassava with Lactobacillus plantarum, Saccharomyces cereviseae, and Rhizopus oryzae on the chemical composition of their flour. International Food Research Journal 22 (3): 1280-1287.
Jayus, Nurhayati, N., Subagio, A., dan Widyatmoko, H. (2016). Modifikasi pati ubi kayu secara fermentasi dengan Lactobacillus manihotivorans dan L. fermentum yang diisolasi dari gatot. Prosiding Seminar Nasional BALITKABI Tahun Anggaran 2016.
Jimoh, S. O., Ado, S. A., Ameh, J. B., Whong, C. M. Z., dan Anglais, A. T. E. (2012). Charateristics and diversity of yeast in locally fermented beverages sold in Nigeria. World of Engineering and Pure and Applied Sci 2 (2): 40-44.
Katekhong, W., dan Charoenrein, S. (2012). Effect of rice storage on pasting properties, swelling and granular morphology of rice flour. Asian Journal of Food and Agro-Industry, 5 (4), 315-321.
Kustyawati, M. E., Sari, M., dan Haryati, T. (2013). Efek fermentasi dengan Saccharomyces cereviseae terhadap karakteristik biokimia tapioka. Agritech 33(3): 281-287.
Maribel, O. M., Senay, S., Orhan, D., dan Kadir, G. G. U. G. (2014). Physicochemical properties of starch from a cereal-based fermented food (Tarhana). Journal of Nutrition & Food Sciences 4 (3): 2-7.
Nurhayati, N., Jenie, B. S. L., Widowati, S., dan Kusumaningrum, H. D. (2014). Komposisi Kimia dan Kristalinitas Tepung Pisang Termodifikasi Secara Fermentasi Spontan dan Siklus Pemanasan Bertekanan-Pendinginan. Agritech, 34 (2), 146-150.
Ogunnaike, A. M., Adepoju, P. A., Longe, A. O., Elemo, G. N., dan Oke, O. V. (2015) Effects of submerged and anaerobic fermentations on cassava flour (Lafun). African Journal of Biotechnology 14 (11): 961-970.
Oslan, S. N., Salleh, A. B., Noor, R., Raja, Z., Rahman, A., Leow, A., dan Chor, T. (2012). Locally isolated yeast from Malaysia: Identification, phylogenetic study and characterization. ACTA BiochemicaPolonica 59 (2): 225-229.
Qi, Y., Cui, T., Jing, Y., Shan, C., Zhao, Z., Wu, P., dan Zhang, X. (2015). Comparative studies between physicochemical properties of white waxy wheat flour and glutionous rice flour. OALib 2 (6): 1-8.
Rahmiati, T. M., Purwanto, Y. A., Budijanto, S., dan Khumaida, N. (2016). Sifat fisikokimia tepung dari 10 genotipe ubi kayu (Manihot esculenta Crantz) hasil pemuliaan. Agritech 36 (4): 459-466.
Schwan, R. F., Almeida, E. G., Souza-dias, M. A. G., dan Jespersen, L. (2007). Yeast diversity in rice-cassava fermentations produced by the indigenous Tapirape people of Brazil. FEMS Yeast Research 7 (1): 966-972.
Subagio, A. (2013). Keunggulan mocaf sebagai bahan baku snack. Foodreview Indonesia 8 (10): 38-40.
Tamang, J. P., Shin, D., Jung, S., dan Chae, S. (2016). Functional properties of microorganisms in fermented foods. Frontiers in Microbiology 7 (4): 1-13.
Wahyuni, S., Ansharullah, Saefuddin, Holilah., dan Asranudin. (2017). Physico-chemical properties of Wikau maombo flour from cassava (Manihot esculenta Crantz). Journal of Food Measurement and Characterization 11 (1): 329-336.
Wang, S., dan Copeland, L. (2013). Molecular disassembly of starch granules during gelatinization and its effect on starch digestibility: a review. Foood & Function 4 (1).
Yuliana, Nety N. S., Sugiharto, R., dan Amhety, D. (2014). Effect of spontaneous lactic acid fermentation on physico-chemical properties of sweet potato flour. Microbiology Indonesia 8 (1): 1-8.
Zavareze, E. da R., Storck, C. R., de Castro, L. A. S., Schrirmer, M. A., dan Dias, A. R. G. (2010). Effect of heat moisture treatment on rice starch of varying amylose content. Food Chemistry 121 (2): 358-365.
Zeng, F., Ma, F., Kong, F., Gao, Q., dan Yu, S. (2015). Physicochemical properties and digestibility of hydrothermally treated waxy rice starch. Food Chemistry 172 (1).
Zhu, F. (2015). Composition, structure, physicochemical properties, and modifications of cassava starch. Carbohydrate polymers. 122: 456-480. DOI: https://doi.org/10.22146/agritech.26323
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