Studi Komparasi: Produksi Bioetanol Nira Batang Kelapa Sawit oleh Flokulan dan Non- Flokulan Saccharomyces cerevisiae

https://doi.org/10.22146/agritech.40938

Kafidul Ulum(1*), Indria Purwantiningrum(2), Retno Dwi Yustina(3), Untung Murdiyatmo(4), Agustin Krisna Wardani(5)

(1) Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya, Jl. Veteran, Malang 65145
(2) Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya, Jl. Veteran, Malang 65145
(3) PT. Sampoerna Agro, Tbk, Jl. Basuki Rachmat No.788, Talang Aman, Kemuning, Kota Palembang, Sumatera Selatan 30127
(4) Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya, Jl. Veteran, Malang 65145
(5) Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya, Jl. Veteran, Malang 65145
(*) Corresponding Author

Abstract


Two types of yeast were used for bioethanol production from oil palm trunk sap, the flocculant Saccharomyces cerevisiae NCYC­1195 and non­flocculant Saccharomyces cerevisiae Kyokai 7 (NCYC-479). Flocculant Saccharomyces cerevisiae is yeast that has ability to aggregate into flocks which precipitate rapidly in culture medium. The effect of urea as a nitrogen source was also investigated in this study. Some concentrations of urea were added i.e. 0%, 0.1%, 0.2%, and 0.3% (w/v) during fermentation. The purpose of this study is to obtain the best condition by strain of Saccharomyces cerevisiae and urea concentration for the highest ethanol production. The highest ethanol production and yield was obtained at 4.86% (v/v) and 0.52 (g/g) respectively, by nonflocculant Saccharomyces cerevisiae Kyokai 7 (NCYC-479) without the addition of urea.

Keywords


Bioethanol; oil palm trunk sap; Saccharomyces cerevisiae; urea



References

Ain, N. (2010). Study on Bioethanol Productionfrom Oil Palm Trunk Sap. Faculty of Chemical & Natural Resources Engineering. Universiti Malaysia Pahang. Malaysia.

Alauddin, Z.A.B.Z., Lahijani P., Mohammadi M., and Mohamed A.R. 2010. Gasification of lignocellulosic biomass in fluidized bed for renewable energy development: a review. Renewable and Sustainable Energy Reviews. 14: 2852-2862.

Aisyah, S.N dan Sembiring, K.C. 2009. Bioproses dan Teknologi Pembuatan Bioetanol. Berita IPTEK LIPI. Tahun ke-47(1): 64-71.

Anonymous. 2013. Ethanol Fuel. http://en.wikipedia.org. Diakses tanggal 16 Agustus 2016.

Apriyantono, A., D. Fardiaz, N. L. Puspitasari, Sedarnawati dan Budiyanto.1989. Analisa Pangan. PAU Pangan dan Gizi. IPB. Bogor Azhar, S H M., Abdulla R, JamboSA., Marbawi H, Gansau J A., Faik A A M., Rodrigue, K F. 2017. Yeasts in sustainable bioethanol production: A review. Biochemistry and Biophysics Reports. Biochemistry and Biophysics. 10:52-61.

Badan Pusat Statistik. Perkembangan Jumlah Kendaraan Bermotor menurut Jenis tahun 1987-2012. www.bps.go.id. Diakses tanggal 23 Oktober 2017.

Badan Pusat Statistik. Produksi Bahan Bakar Minyak 1996-2017. https://www.bps.go.id/dynamictable/2016/01/28/1125/produksi-bahan-bakar-minyak-bbm-1996-2015.html. Diakses tanggal 3 Juli 2019.

Bai, F.W, W.A. Anderson, M. Moo-Young. 2008. Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnology Advances. Vol. 26:89–105.

Baig MMV, Mane VP, More DR, Shinde LP, Baig MIA. 2003. Utilization of
Agricultural Waste of Banana: Production of Cellulases by Soil fungi, J. Environ. Biol. 24:173 -176.

Domingues.L, A.A Vicente, N. Lima, J.A Teixeira. 2000. Applications of yeast flocculation in biotechnological processes. Biotechnol. Bioprocess Eng. Vol.5: 288-305.

Demain, A.L., Newcomb, M., and Wu, J.H.D. 2005. Cellulase, Clostridia, and
Ethanol. Microbiology and Molecular Biology Reviews. 69: 124-154

Direktorat Jenderal Minyak dan Gas Bumi Kementerian Energi dan Sumber Daya Mineral 2017. Statistik Minyak dan Gas Bumi Tahun 2016. Jakarta.

Erten H., H. Tanguler, T. Cabaroglu., A. Canbas. 2006. The Influence of Inoculum Level on Fermentation and Flavour Compounds of White Wines Made from cv. Emir. J. Inst. Brew. 112(3):232-236

Forgacs, E. And Cserhati, T. 2003. Gas Chromatography. Woodhead Publishing Limited and CRC Press LLC. New York

Gancedo, J.M. 1998. Yeast Carbon Catabolite Repression. Microbiology and Molecular Biology Reviews. Vol. 62(2): 334-361.

Guritno P, Darnoko D. 2003. Teknologi Pemanfaatan Limbah Dari Peremajaan Perkebunan Kelapa Sawit. Seminar Nasional: Mengantisipasi Regenerasi Pertama Perkebunan Kelapa Sawit di Indonesia 9 – 10 April 2003. Bali: Max Havelaar Indonesia Foundation.

Harris, Sjamsjul Anam, dan Syarifuddin Mahmudsyah.2013. Studi Pemanfaatan Limbah Padat dari Perkebunan Kelapa Sawit pada PLTU 6 MW di Bangka Belitung. Jurnal Teknik Pomits Vol. 2, No. 1, (2013) ISSN: 2337-3539.

Hendriksen, H.V. and Ahring, B.K. 1991. Effects of ammonia on growth and morphology of thermophilic hydrogen-oxidizing methanogenic bacteria. FEMS Microbila Ecology. 85: 241-246

Hermawan, D. R. W. A., Utami., T. dan Cahyanto, M. N., 2000. Fermentasi
Etanol dari Buah Semu Jambu Mete (Anacardium occidentale L.)
oleh Saccharomyces cereviseae FNCC 3015 Menggunakan
Ammonium Sulfat dan Urea Sebagai Sumber Nitrogen. Agritech. 20(2): 93 – 98.

Indartono, Y. 2005. Bioetanol Alternatif Energi Terbarukan: Kajian Prestasi Mesin dan Implementasi di Lapangan. www.energi.lipi.go.id. Diakses tanggal 26 september 2016.

Jennifer, C.B. 2005. Characteristics of Flo11-dependent Flocculation in Saccharomyces cerevisiae. FEMS Yeast Res. 5 (2005) 1151–1156.

Kida, K., Morimura S., Kume K., Suruga K., and Sonoda Y. 1991. Repeated
batch ethanol fermentation by a flocculating yeast Saccharomyces
cerevisiae IR-2. J. Ferment Bioeng. 71:340–344.

Khongsay, N., Lakkana, L. And Pattana, L. 2010. Growth and Batch Ethanol
Fermentation of Saccharomyces cerevisiae on Sweet Sorghum Stem
Juice under Normal and Very High Gravity Condition. Biotechnol. 9(1): 9-16

Kosugi, A., Tanaka, R., Magara, K., Murata, Y., dkk. 2010. Ethanol and lactic acid production using sap squeezed from oil palm trunks felled for replanting, Journal of Bioscience and Bioengineering, vol. 110, no. 3, pp. 322- 325, 2010.

Lin, Y.H, W.S Chien, K.J Duan, and P.R Chang. 2011. Effect of Aeration Timing and Interval During Very High Gravity Ethanol Fermentation. Process Biochemistry. Vol. 46:1025-1028.

Madigan, M.T, Martinko, J.M, and Parker, J. 2000. Nutirition and metabolism Brock biology of microbiology. 9th. ed. NJ: prentice-Hall.

Maurice, M. L. 2011. Factors Effecting Ethanol Fermentation Via Simultaneous Saccharification and Fermentation. Major Qualifying Project for the Gegree of Bachelor Science.Worcester Polytechnic Science.

Maiorella. 1985. Ethanol. Comprehensive Biotechnology. Vol.3 Pergamon.

Michael, H. L., W. R. James., L. O Joe., T. H. Mark, and F.O. Rene. 2006. The Economics of Ethanol from Sweet Sorghum Using the MixAlco Process. http://www.afpc.tamu.edu/pubs/2/446/RR%2006-2.pdf. Tanggal akses 20 Agustus 2014.

Miki, B.L.A., Poon, N.H., James, A.P., Seligy, V.L. 1982. Possible mechanism for flocculation interactions governed by gene FLO1 in Saccharomyces cerevisiae. J Bacteriol 150,878–889

Miller, G.L. 1959. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal Chem. 31: 426.

Mori, Yutaka.2007. Old Oil Palm Trunks as Promising Feedstock for Biofuel and Bioplastics. University of Science Malaysia and Forest Research Institute Malaysia. 4th BMWS 2007.

Mukhtar, K., M. Asgher, S. Afghan, K. Hussain, and S. Zia-ul-Hussnain. 2010.
Comparative Study on Two Commercial Strains of Saccharomyces
cerevisiae for Optimum Ethanol Production on Industrial Scale. J. Biomed. Biotechnol. 2010: 5

Naik, S.N., V.V. Goud, P.K. Rout., A.K. Dalai. 2010. Production of first and second generation biofuels: A comprehensive review. Renewable and Sustainable Energy Reviews. Vol. 14: 578-597.

Nofemele, Z, Pratyoosh Shukla, Arthur Trussler, Kugen Permaul, and Suren Sigh. 2012. Improvement of Ethanol Production from Sugarcane Molasses through enhanched nutrient Supplementation using Saccharomyces cerevisiae. Journal of Brewing and Distillatilling 3(2): 29-35.

Quintero, J.A., V. 2006. Palm sugar: the Indigeneous Sweetness. ILEIA Newslett. 13(2): 19

Richana, Nur dan Suarni. 2008. Teknologi Pengolahan Jagung. Balai Besar Penelitian dan Pengembangan Pasca Panen. Bogor.

Samejima, M. 2008. Scenario of Technical Innovation for Production of Ethanol as Automobile Fuel from Cellulosic Biomass in Japan. Proceedings International Symposium on Wood Scienceand Technology. Harbin, China, 27-29 Sep 2008. Harbin: Iternational Association of Wood Products Societies.

Soares, E.V. 2010. Flocculation of Saccharomyces cerevisiae : a Review. Journal of Applied Microbiology ISSN 1364-5072. Vol.110: 1-18.

Sukumaran, R.K., dkk. 2008. Cellulase Production Using Biomassa Feed Stock and Its Application in Lignocellulosa Saccharification for Bioethanol Production. Renewable Energy.Vol. 30: 1-4.

Sultana, S., Mohd Jamil1, N., Saleh, E A M, Yousuf, A., and Faizal, C.K.M. 2017. A mathematical model for ethanol fermentation from oil palm trunk sap using Saccharomyces cerevisiae. J. Phys.: Conf. Ser. 890 012050. 1-6.

Suriyachai N., K. Weerasaia, N. Laosiripojana, V. Champreda, 2013. Optimized simultaneous saccharification and co-fermentation of rice straw for ethanol production by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture using design of experiments. Bioresource technology 142: 171-178.

Tohari, A. 2012. Sekali lagi: Etanol dari Tebu. P3GI. Pasuruan.

Wahyuni. A. 2008. Rekayasa bioproses pembuatan bioetanol dari sirup glukosa ubi jalar (Ipomoea batatas L) dengan menggunakan Saccharomyces cerevisiae.Thesis.Sekolah pasca Sarjana.IPB. Bogor.

Waites, MJ, Morgan, NL, John, SR, Gary, H. 2001. Industrial Microbiology an Introduction. Blackwell Publishing Company, Victoria Australia.

Wyman, C.E. 1999. Ethanol Production From Lignocellulosic Biomass: overview. CRC Press. Inc. Cleceland Ohio. Chapter 1:1-18

Yamada H., Tanaka R., Sulaiman O, Hashim R., Hamid, Z.A.A., Yahya M.K.A, Kosugi, A., Arai T. Murata Y., Nirasawa S, Yamamoto K., Ohara S., Yusof M N M., Ibrahim, W A., Mori Y. 2010. Old oil palm trunk: A promising source of sugars for bioethanol production. Biomass and Bioenergy. 34(11):1608-1613.



DOI: https://doi.org/10.22146/agritech.40938

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Copyright (c) 2021 Kafidul Ulum, Indria Purwantiningrum, Retno Dwi Yustina, Untung Murdiyatmo, Agustin Krisna Wardani

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