Effect of Partially Purified Polygalacturonase and Cellulase on Red Guava Juice Clarification at Various Incubation Times and Temperatures

https://doi.org/10.22146/agritech.52516

Esti Widowati(1*), Adhitya Pitara Sanjaya(2), Ardhea Mustika Sari(3), Shindy Ambarwati(4)

(1) Department of Food Science Technology, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Kentingan, Jebres, Surakarta 57126
(2) Department of Food Science Technology, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Kentingan, Jebres, Surakarta 57126
(3) Department of Food Science Technology, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Kentingan, Jebres, Surakarta 57126
(4) Department of Food Science Technology, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Kentingan, Jebres, Surakarta 57126
(*) Corresponding Author

Abstract


Red guava juice has some undesirable characteristics including high viscosity and cloudiness as well as sediment formation due to the presence of polysaccharide in the juice. Hence, enzymatic treatment for juice clarification is needed to overcome this problem. The current study was aimed to evaluate the effect of incubation times and temperature on juice clarification using partially purified polygalacturonase from Bacillus licheniformis and cellulase from Bacillus subtilis. The incubation times of 60,90, and 120 minutes and temperatures of 35 °C, 47.5 °C, and 60 °C were used in this study. pH value, total dissolved solids (TDS), transmittance, viscosity, and yield were analyzed. The results showed that incubation temperature had a significant effect on all the parameters except for pH value. The interaction between incubation temperature and time on decreasing pH value and viscosity was observed. The best clarification condition was observed at incubation time of 47.5 °C for 90 minutes, which resulted in pH, TDS, transmittance, viscosity, and yield of 4.98±0.13, 7.67±0.21 °Brix, 23.27±0.24%T, 36.37±3.46 cP, and 77.51±1.95% respectively.

Keywords


Cellulase; Clarification; Incubation; Polygalacturonase; Red Guava Juice

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References

Ahmed, B., Uddin, M. B., & Jubayer, M. F. (2014). Extraction and Standardization of Selected Fruit Juices by Enzymatic Process. Peak Journal of Food Science and Technology,2, 18-27.

Akesowan, A., & Choonhahirun, A. (2013). Effect of Enzyme Treatment on Guava Juice Production UsingResponse Surface Methodology. J. Anim. Plant Sci 23(1): 114-120.

Arsad, P., Sukor, R., Wan Ibadullah, W. Z., Mustapha, N.A., & Meor Hussin, A. S. (2015). Effects of Enzymatic Treatment on Physicochemical Properties of Sugar Palm Fruit Juice. International journal on advanced science engineering information technology,5(5),308-312. https://doi.org/10.18517/ijaseit.5.5.577.

Barbalho, A. M., Farinazzi-Machado, F. M. V., Goulart, R.A., Brunnati, A. C. S., Machado, A. M., Ottoboni, B.,& Nicolau, C. S. T. (2012). Psidium guajava (Guava): A Plant of Multipurpose Medicinal Applications. Med Aromat Plants, 4(1): 1-6. https://doi.org/10.4172/2167-0412.1000104.

Bonnin, E. & Lahaye, M. (2013). Survey Contribution of Cell Wall-Modifying Enzymes to The Texture of Fleshy Fruits.The Example of Apple. J. Serb. Chem. Soc, 78 (3), 417–427. https://doi.org/10.1145/2652481.

Carvalho, L. M. C & Silva, C. A. B. (2010). Clarification of Pineapple Juice by Microfiltration. Ciênc. Tecnol. Aliment., Campinas, 30(3), 828-832. https://doi.org/10.1590/ S0101-20612010000300040.

Diniz, R. S., Coimbra, J. S. R., Martins, M. A., Santos, M. O., Diniz, M. D. M. S., Santos, E. S., Santánna, D. D., Rocha, R. A., & Oliveira, E. B. (2015). Physical Properties of Red Guava (Psidium guajava L.) Pulp as Affected by Soluble Solids Content and Temperature. International Journal of Food Engineering, 10(3), 437–445. https:// doi.org/10.1515/ijfe-2012-0250.

Golan, A. E. (2011). Cellulase: Types and Action, Mechanism and Uses. New York: Nova Science Publishers, Inc.

Izadi, A., Rashedi, H., Ghafarzadegan, R., & Hajiaghaee, R. (2015). Evaluation the Effect of Enzymatic Process on the Edible Aloe Vera Gel Viscosity Using Commercial Cellulase. Journal of Applied Biotechnology Reports,2(3), 299-303.

Jori, D. B., Pawar, A. V., Kudake, D. C., & Kotgirkar, P. G. (2015). Multienzymatic Clarification of Blended Pineapple and Mango Pulp Using Response Surface Methodology. International Journal of Advanced Biotechnology and Research, 6, 49-56. http://doi.org/10.35940/ijtee. L3237.1081219.

Kaur, S., Sarkar, B. C., Sharma, H. K., & Singh, C. (2011).Response Surface Optimization of Conditions for the Clarification of Guava Fruit Juice using Commercial Enzyme. Journal of Food Process Engineering, 34(4). https://doi.org/10.1111/j.1745-4530.2009.00414.x.

Kumar, S. (2015). Role of Enzymes in Fruit Juice Processing and Its Quality Enhancement. Advances in Applied Science Research, 6(6), 114-124.

Le, T. T., Nguyen, V. P. T., & Le, V. V. M. (2012). Application of Cellulase Preparation to Guava Mash Treatment in Juice Processing: Optimization of Treatment Conditions by RSM. As. J. Food Ag-Ind ,5(04), 284-291.

Nakkeeran, E., Kumar, S. U., & Subramanian, R. (2011).Aspergillus carbonarius Polygalacturonases Purified by Integrated Membrane Process and Affinity Precipitation for Apple Juice Production. Bioresource Technology, 102(3), 3293-3297.https://doi.org/10/1016/j. biortech.2010.10.048.

Ninga, K. A., Sengupta, S., Jain, A., Desobgo, Z. S. C., Nso, E. J., & De, S. (2018). Kinetics of Enzymatic Hydrolysis of Pectinaceous Matter in Guava Juice. Journal of Food Engineering, 221, 158-166. https://doi.org/10.1016/j. foodeng.2017.10.022.

Ongratto, R. S. & Viotto, L. A. (2016). Enzyme Optimization to Reduce the Viscosity of Pitanga (Eugenia uniflora L.) Juice. Brazilian Journal of Food Technology, 19, 1-8. http://dx.doi.org/10.1590/1981-6723.4115.

Robin., S. K., Singh, D., & Sharma, H. K. (2013). Optimization of Enzymatic Hydrolysis Conditions for Enhanced Juice Recovery with Optimum Quality from Alu Bukhara (Prunus domestica L.) Fruit. International Journal of Advanced Research in Engineering and Applied Sciences,2(11), 50-67.

Sassi, A. H., Tounsi, H., Trigui-Lahiani, H., & Bouzouita, R. (2016). A Low-Temperature Polygalacturonase from P.Occitanis: Characterization and Application in Juice Clarification. International Journal of Biological Macromolecules, 91, 158-164. http://doi.org/10.1016/j. ijbiomac.2016.05.075.

Sharma, H. P., Patel, H., & Sharma, S. (2014). Enzymatic Extraction and Clarification of Juice from Various Fruits-A Review. Trends in Postharvest Technology, 2(1), 1-14. https://doi.org/10.1080/10408398.2014.977434.

Sharma, H. P., Sharma, S., Vaishali., & Prasad, K. (2015).Application of Non Thermal Clarification in Fruit Juice processing - A Review.South Asian J. Food Technol. Environ, 1(1), 15- 21.

Sinatari, H. M., Aminin, A. L. N., & Sarjono, P. R. (2013).Pemurnian Selulase dari Isolat Kb Kompos Termofilik Desa Bayat Klaten Menggunakan Fraksinasi Amonium Sulfat. Chem Info, 1(1): 130-140.

Ünal, M. U., & Sener, A. (2015). Extraction and Characterization of Pectin Methylesterase from Alyanak Apricot (Prunus armeniaca L). J Food Science Technology, 52(2), 1194–1199. https://doi.org/10.1007/s13197-013-1099-3.

Widowati, E., Sanjaya, A. P., & Syahiidah, A. (2019). Aplikasi Enzim Poligalakturonase dan Enzim Selulase pada Klarifikasi Sari Buah Jambu Biji Merah (Psidium guajava L.). In Seminar Nasional Dies Natalis ke 43 2019 Universitas Sebelas Maret . Surakarta, Indonesia 3(1), 182.

Widowati, E., Utami, R., & Kaistyatika, K. (2017). Screening and Characterization of Polygalacturonase as Potential Enzyme for Keprok Garut Orange (Citrus nobilis Var. Chrysocarpa) Juice Clarification. Journal of Physics. Conference Series, 909(1), 1-10. https://doi. org/10.1088/1742-6596/909/1/012088.

Widowati, E., Utami, R., Nurhartadi, E., & Putro, R. R. M. (2016).Screening and Characterization of Cellulose Enzyme in Sweet Orange (Citrus sinensis) Juice Clarification. Proceeding. The Sixth Indonesian Biotechnology Conference: 397-403.

Yadav, S. K., Sarolia, D. K., Pilania, S., Meena, H.R., & Mahawer, L. N. (2017). Studies on Keeping Quality of Preserved Guava Pulp during Storage. International Journal of Current Microbiology and Applied Sciences, 6(3), 1235-1242. https://doi.org/10.20546/ijcmas.2017.603.142.




DOI: https://doi.org/10.22146/agritech.52516

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