Aktivitas Hidrolisis Enzim Lipase dari Kentos Kelapa terhadap Minyak Kelapa


Mohammad Su’i(1*), Harijono Harijono(2), Yunianta Yunianta(3), Aulani’am Aulani’am(4)

(1) Fakultas Pertanian, Universitas Widyagama Malang, Jl. Borobudur No. 25, Malang
(2) Fakultas Teknologi Pertanian, Universitas Brawijaya Malang, Jl. Veteran, Malang 65145
(3) Fakultas Teknologi Pertanian, Universitas Brawijaya Malang, Jl. Veteran, Malang 65145
(4) Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya Malang, Jl. Veteran, Malang 65145
(*) Corresponding Author


This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil) concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show  that lipase of coconut houstorium may be optimally used at a coconut oil concentration of 10 %, enzyme to substrate ratio of 3 : 10 (v/v) and hydrolysis for 60 minutes with stirring.


Penelitian ini mempelajari kondisi hidrolisis minyak kelapa yang optimum menggunakan enzim lipase dari kentos kelapa. Kondisi hidrolisis yang dipelajari meliputi konsentrasi substrat optium, perbandingan enzim : substrat dan lama hidrolisis yang optimum serta pengaruh pengadukan selama hidrolisis. Hasil penelitian menunjukkan bahwa, hidrolisis minyak kelapa menggunakan enzim lipase kentos kelapa menghasilkan asam lemak bebas paling banyak pada kon- sentrasi substrat (minyak kelapa) 10 %, perbandingan enzim : substrat yaitu 3 : 10 (v/v), lama hidroloisa 60 menit dan dilakukan pengadukan selama hidrolisis.


Lipase; houstorium; coconut oil; hydrolysis

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DOI: https://doi.org/10.22146/agritech.9669

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agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.

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