* Corresponding Author
Aktivitas Antioksidan Minuman Daun Beluntas Teh Hitam (Pluchea indica Less-Camelia sinensis)
https://doi.org/10.22146/agritech.25699 Paini Sri Widyawati(1*), Tarsisius Dwi Wibawa Budianta(2), Yesiana Dwi Wahyu Werdani(3), Maria Olivia Halim(4)
(1) Program Studi Teknologi Pangan, Fakultas Teknologi Pertanian, Universitas Katolik Widya Mandala Surabaya, Jl. Dinoyo 42-44 Surabaya, 60265
(2) Program Studi Teknologi Pangan, Fakultas Teknologi Pertanian, Universitas Katolik Widya Mandala Surabaya, Jl. Dinoyo 42-44 Surabaya, 60265
(3) Fakultas Keperawatan, Universitas Katolik Widya Mandala Surabaya, Jl. Kalisari Selatan 1, Pakuwon City, Surabaya, 60112
(4) Program Studi Teknologi Pangan, Fakultas Teknologi Pertanian, Universitas Katolik Widya Mandala Surabaya, Jl. Dinoyo 42-44 Surabaya, 60265
(*) Corresponding Author
Abstract
The research was conducted to explore the potency of pluchea leaves-black tea drink as antioxidant at various proportions. The research used a single factor randomized block design of pluchea leaves and black tea proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w). Each of it was repeated five times. The parameters observed in this study were secondary metabolites, total phenolic, total flavonoids, free radical DPPH scavenging activity, and iron reducing power. The data were statistically analyzed using Analysis of Variance (ANOVA) at α = 5%, if the analysis showed a significant effect then it was continued with Duncan's Multiple Range Test (DMRT). Data was stated as mean ± standard deviation. The results showed that the secondary metabolites containing in drink from pluchea leaves and black tea at various proportions were alkaloids, flavonoids, phenolics, saponins, tannins, and cardiac glycosides. The increasing of black tea proportion in samples added the intensity of alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides compounds detected, but the tannins were decreased. These secondary metabolites were correlated with total phenolic content (TPC) and total flavonoid content (TFC). The increasing of black tea proportion in drink significantly decreased DPPH free radical scavenging activity and iron ion reducing power, except for 100% black tea proportion. Tannin compounds seems determining antioxidant activity. Based on coefficient correlation between TPC or TFC and DPPH free radical scavenging activity or iron ion reducing power, the result showed that DPPH free radical scavenging activity was dominantly contributed by TPC and iron ion reducing power was determined by TPC and TFC.
ABSTRAK
Penelitian ini dilakukan untuk mengetahui potensi minuman daun beluntas teh hitam sebagai antioksidan pada berbagai proporsi. Penelitian ini menggunakan rancangan acak kelompok satu faktor, yaitu proporsi daun beluntas teh hitam meliputi 100:0; 75:25; 50:50; 25:75; dan 0:100% (b/b). Tiap faktor diulang sebanyak 5 kali. Parameter yang diamati pada penelitian ini meliputi kandungan metabolit sekunder, total fenolik (TPC), total flavonoid (TFC), kemampuan menangkal radikal bebas DPPH, dan kemampuan mereduksi ion besi. Data dianalisis secara statistik dengan Analysis of Variance (ANOVA) pada α = 5%, jika terdapat beda signifikan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Data dinyatakan dengan rata-rata ± standar deviasi. Hasil menunjukkan bahwa metabolit sekunder yang terkandung dalam minuman daun beluntas teh hitam pada berbagai proporsi adalah alkaloid, flavonoid, fenolik, saponin, tannin, dan kardiak glikosida. Peningkatan proporsi teh hitam menambah intensitas senyawa alkaloid, fenolik, flavonoid, saponin, dan kardiak glikosida yang terdeteksi, tetapi kandungan senyawa tannin berkurang. Kandungan metabolit sekunder ini berkorelasi dengan total fenolik (TPC) dan total flavonoid (TFC). Peningkatan proporsi teh hitam dalam minuman menurunkan kemampuan menangkal radikal bebas DPPH dan kemampuan mereduksi ion besi, kecuali pada proporsi teh hitam 100%. Kandungan senyawa tannin dalam minuman menentukan aktivitas antioksidan. Berdasarkan koefisien korelasi antara TPC atau TFC dan kemampuan menangkal radikal bebas DPPH atau kemampuan mereduksi ion besi, hasil menunjukkan bahwa kemampuan menangkal radikal bebas DPPH dominan dikontribusi oleh TPC dan kemampuan mereduksi ion besi ditentukan oleh TPC dan TFC.
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Al-Temimi, A., & Choudhary, R. (2013). Determination of antioxidant activity in different kinds of plants in vivo and in vitro by using diverse technical methods. Journal Nutrition of Food Science, 3(1),1-9. http://doi.org/10.4172/2155-9600.1000184.
Amic, D., Davidovic-Amic, D., Beslo, D., & Trinajsti, N. (2003). Structure-radical scavenging activity relationships of flavonoids. Croatica Chemica Acta, 76(1), 55-61. doi:10.1016/j.phytochem.2006.07.002.
Andarwulan, N., Batari, R., Sandrasari, D. A., Bolling, B., & Wijaya, H. (2010). Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chemistry, 121. 1231–1235. doi:10.1016/j.foodchem.2010.01.033.
Ardiansyah, Nuraida, L., & Andarwulan, N. (2003). Aktivitas antimikroba daun beluntas (Pluchea indica Less) dan stabilitas aktivitasnya pada berbagai konsentrasi garam dan tingkat pH. Jurnal Teknologi dan Industri Pangan, 14(2), 90-97.
Ashok, P.K., & Upadhyaya, K. (2012). Tannins are astringent. Journal of Pharmacognosy and Phytochemistry, 1(3), 45-50.
Atanassova, M., Georgieva, S., & Ivancheva, K. (2011). Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of The University of Chemical Technology and Metallurgy, 46(1), 81-88.
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und-Technology, 28, 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5.
Cabrera, C., Reyes, A., & Rafael, G. (2006). Beneficial effects of green tea: A review. Journal of the American College of Nutrition, 25(2), 79-99. https://doi.org/10.1080/07315724.2006.10719518
Chanda, S., & Dave, R. (2009). In vivo models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An Overview. African Journal of Microbiology Research, 3(13), 981-996.
Deetae, P., Parichanon, P., Trakunleewatthana, P., Chanseetis, C., & Lertsiri, S. (2012). Antioxidant and anti-glycation properties of Thai herbal teas in comparison with conventional teas. Food Chemistry, 133(3), 953–959. https://doi.org/10.1016/j.foodchem.2012.02.012
Frutos, P., Hervás, G., Giráldez, F.G., & Mantecón, A.R. (2004). Revew. Tannins and Ruminant Nutrition. Spanish Journal of Agricultural Research, 2(2), 191-202.
Gardjito, M., & Rahadian, D. (2015). Teh. Yogyakarta: PT Kanisius.
Harborne, J. B. (1996). Phytochemical Method. Padmawinata K and Soediro I (Eds.). Bandung : Institut Teknologi Bandung.
Kamtekar, S., Keer, V., & Patil, V. (2014). Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. Journal of Applied Pharmaceutical Science, 4 (09), 061-065. http://doi.org/10.7324/JAPS.2014.40911
Karori, S., Ngure, R.M., Wachira, F.N., Wanyoko, J.K., & Mwangi, J.N. (2008). Different types of tea products attenuate inflammation induced in Trypanosoma brucei brucei-infected mice. Parasitology International, 57(3), 325-333.http://doi: 10.1016/j.parint.2008.02.003
Keerthana, G., Kalaivani, M.K., & Sumathy, A. (2013). In-Vitro alpha amylase inhibitory and anti-oxidant activities of ethanolic leaf extract of Croton Bonplandianum. Asian Journal of Pharmaceutical and Clinical Research, 6(4), 32-36. http://dx.doi.org/10.22159/ijcpr.2017v9i2.17379
Leenen, R., Roodenburg, A.J.C., Tijburg, L.B.M., & Wiseman, S.A. (2000). A single dose of tea with or without milk increases plasma antioxidant activity in humans. European Journal of Clinical Nutrition, 54(1),87-92. http://doi.org/10.1038/sj.ejcn.1600900
Maurya, P.K., & Rizvi, S.I. (2009). Protective role of tea catechins on erythrocytes subjected to oxidative stress during human aging. Natural Product Research, 23(12),1072-1079. https://doi.org/10.1080/14786410802267643
Mohan, Y., Jesuthankaraj, G.N., & Thangavelu, N.R. (2013). Antidiabetic and antioxidant properties of Triticum aestivum in streptozotocin-induced diabetic rats. Advances in Pharmacological Sciences, 23(12),1072-1079. http://dx.doi.org/10.1155/2013/716073
Obanda, M., Owuor, P.O., Mang’oka, R., & Kavoi, M.M. (2004). Changes in thearubigin fractions and theaflavin levels due to variations in processing conditions and their influence on black tea liquor brightness and total colour. Food Chemistry, 85(2),163-173. https://doi.org/10.1016/S0308-8146(02)00183-8
Pontis, J.A., Costa, L.A.M.A., Silva, S.J.R., & Flach, A. (2014). Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil. Food Science and Technology Campinas, 34(1), 69-73. http://dx.doi.org/10.1590/S0101-20612014005000015.
Rebaya, A., Belghith, S.I., Baghdikian, B., Leddet, V.M., Mabrouki, F., Olivier, E., Cherif, J.K., & Ayadi, M.T. (2014). Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae). Journal of Applied Pharmaceutical Science, 5(01),052-057. http://doi.org/10.7324/JAPS.2015.50110
Rohman, A., Riyanto, S., Yuniarti, N., Saputra, W. R., Utami, R., & Mulatsih, W. (2010). Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). International Food Research Journal, 17, 97-106.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin ciocalteu reagent. Methods in Enzymology, 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
Song, J.M., Lee, K.H., & Seong, B.L. (2005). Antiviral effect of catechins in green tea on influenza virus. Antiviral Research, 68(2), 66-74. https://doi.org/10.1016/j.antiviral.2005.06.010
Srisook, K., Buapool, D., Boonbai, R., Simmasut, P., Charoensuk, Y., & Srisook, E. (2012). Antioxidant and anti-inflammatory activities of hot water extract from Pluchea indica Less herbal tea. Journal of Medicinal Plants Research, 6(23),4077-4081. https://doi.org/10.5897/JMPR12.773
Stanojević, L., Stanković, M., Nikolić, V., Nikolić, L., Ristić, D., Brunet, J.C., & Tumbas, V. (2009). Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella l. extracts. Sensors, 9(7), 5702-5714. https://doi.org/10.3390/s90705702
Tapas, A., Sakarkar, D.M., & Kakde, R.B. (2008). Flavonoids as nutraceuticals: a review. Tropical Journal of Pharmaceutical Research, 7(3), 1089-1099.
Tinkílíç, N., & Uyaník, A. (2001). Spectrophotometric determination of the tannin contents of various Turkish black tea, beer and wine samples. International Journal of Food Sciences and Nutrition, 52(3), 289-294. https://doi.org/10.1080/09637480120044147
Widyawati, P. S., Wijaya, C. H., Hardjosworo P.S., & Sajuthi, D. (2011). Evaluasi aktivitas antioksidatif ekstrak daun beluntas (Pluchea indica Less) berdasarkan perbedaan ruas daun. Rekapangan Jurnal Teknologi Pangan, 5(1),1-14.
Widyawati, P.S., Wijaya, C.H., Hardjosworo, P.S., & Sajuthi, D. (2012). Antioxidant activity of pluchea (Pluchea indica Less) leaves methanolic extract and its fractions. Agritech, 32(3), 249-257. https://doi.org/10.22146/agritech.9618
Widyawati, P.S., Budianta, T.D.W., Kusuma, F.A., & Wijaya, E.L. (2014a). Difference of solvent polarity to phytochemical content and antioxidant activity of Pluchea indica Less leaves extracts. International Journal of Pharmacognosy and Phytochemical Research, 6(4), 850-855.
Widyawati, P.S., Budianta, T.D.W., Kusuma, F.A., Wijaya, E.L., Yaunatan, D.I., & Wongso, R.S. (2014b, November). Potency of beluntas (Pluchea indica Less) leaves extract as antioxidant and anti warmed over flavor (WOF) of duck meat. International congress challenges of biotechnological research in food and health, Surakarta, Indonesia.
Widyawati, P.S. (2016a). Determination of antioxidant capacity in Pluchea indica Less leaves extract and its fractions. International Journal of Pharmacy and Pharmaceutical Sciences, 8(9), 32-36. https://dx.doi.org/10.22159/ijpps.2016v8i9.11410
Widyawati, P.S., Budianta, T.D.W., Utomo, A.R., & Harianto, I. (2016b). The physicochemical and antioxidant properties of Pluchea Indica Less drink in tea bag packaging. International Journal of Food and Nutritional Sciences, 5(3), 113-120.
Yam, T.S., Shah, S., & Hamilton-Miller, J.M.T. (1997). Microbiological activity of whole and fractionated crude extracts of tea (Camellia sinensis), and of tea components. FEMS Microbiology Letters, 152(1),169-174. https://doi.org/10.1111/j.1574-6968.1997.tb10424.x
DOI: https://doi.org/10.22146/agritech.25699
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