* Corresponding Author
Whey Protein-Pectin Conjugate by Wet-Dry Heating: Optimization using Response Surface Methodology with Box-Behnken Design
https://doi.org/10.22146/agritech.71301 Elita Yeliani(1), Arima Diah Setiowati(2), Chusnul Hidayat(3*)
(1) Departement of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(2) Departement of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(3) Departement of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281
(*) Corresponding Author
Abstract
The recent progress in glycation of proteins utilizing saccharides through the Maillard reaction has garnered substantial attention, with a specific emphasis on Whey Protein Concentrate (WPC). Conjugation mode is frequently intricate and poses challenges when scaling up for large-scale production. Consequently, this investigation sought to optimize the conditions of the WPC-pectin conjugation process using Response Surface Methodology (RSM) in conjunction with Box-Behnken design (BBD). The experimentation was executed employing a cabinet dryer, incorporating both wet and dry heating procedures to yield a WPC-pectin conjugate exhibiting favorable functional properties. The independent variables investigated encompassed pectin concentration (ranging from 0 to 1%), pH (ranging from 6 to 8), and drying time (ranging from 2 to 6 hours), The measured responses encompassed the emulsion stability index (ESI), emulsifying activity index (EAI), and solubility. Analyzing the experimental data underwent scrutiny for model sufficiency through diagnostic plots, and a second-order polynomial equation was fitted through multi-response regression analysis, resulting in a high coefficient of determination (R 2 ) value. The most effective parameters were identified as a pectin concentration of 0.49%, pH 6.7, and a drying duration of 4.12 hours, yielding a peak ESI of 452.267 minutes, EAI measuring 49.95 m 2 g -1 , and solubility reaching 48.09%. Further experiments were conducted to validate these outcomes, and the presence of the Maillard reaction was confirmed using Fourier Transform Infrared Spectrum (FTIR). The et-dry method demonstrated efficacy in producing WPCpectin conjugates with commendable functional properties.
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A’yun, Q., Azzahrani, I. N., Huyst, A., de Neve, L., Martins, J. C., van Troys, M., Hidayat, C., & Van der Meeren, P. (2020). Heat stable whey protein stabilised O/W emulsions: Optimisation of the whey protein concentrate dry heat incubation conditions. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 603(June), 125192. https://doi.org/10.1016/j.colsurfa.2020.125192
Candraningrum, R. G. S., Setiowati, A. D., & Hidayat, C. (2022). Electrostatic-Maillard formation of coconut protein Concentrate-Pectin conjugate for Oil-in-Water Emulsion: Effects of Ratio, Temperature, and pH. Journal of the Saudi Society of Agricultural Sciences, 22(2023), 18–24. https://doi.org/10.1016/j.jssas.2022.05.004
Chen, W., Lv, R., Wang, W., Ma, X., Muhammad, A. I., Guo, M., & Liu, D. (2019). Time effect on structural and functional properties of whey protein isolate-gum acacia conjugates prepared via Maillard reaction. Journal of the Science of Food and Agriculture, 99(2019), 4801–4807. https://doi.org/10.1002/jsfa.9735
Chen, W., Ma, X., Wang, W., Lv, R., Guo, M., Ding, T., Ye, X., Miao, S., & Liu, D. (2019). Preparation of modified whey protein isolate with gum acacia by ultrasound maillard reaction. Food Hydrocolloids, 95(2018), 298–307.
Dong, X., Du, S., Deng, Q., Tang, H., Yang, C., Wei, F., Chen, H., Quek, S. Y., Zhou, A., & Liu, L. (2020). Study on the antioxidant activity and emulsifying properties of flaxseed gum-whey protein isolate conjugates prepared by Maillard reaction. International Journal of Biological Macromolecules, 153, 1157–1164. https://doi.org/10.1016/j.ijbiomac.2019.10.245
Guo, X., Guo, X., Meng, H., Chen, X., Zeng, Q., & Yu, S. (2019). Influences of different pectins on the emulsifying performance of conjugates formed between pectin and whey protein isolate. International Journal of Biological Macromolecules, 123, 246–254. https://doi.org/10.1016/j.ijbiomac.2018.11.040
Liu, G. & Zhong, Q. (2013). Thermal aggregation properties of whey protein glycated with various saccharides. Food Hydrocolloids, 32(1), 87–96. https://doi.org/10.1016/j.foodhyd.2012.12.008
Nooshkam, M. & Varidi, M. (2020). Maillard conjugate-based delivery systems for the encapsulation, protection, and controlled release of nutraceuticals and food bioactive ingredients: A review. Food Hydrocolloids, 100(June 2019), 105389. https://doi.org/10.1016/j.foodhyd.2019.105389
Pirestani, S., Nasirpour, A., Keramat, J., & Desobry, S. (2017). Preparation of chemically modified canola protein isolate with gum Arabic by means of Maillard reaction under wet-heating conditions. Carbohydrate Polymers, 155, 201–207. https://doi.org/10.1016/j.carbpol.2016.08.054
Sedaghat Doost, A., Nikbakht Nasrabadi, M., Wu, J., A’yun, Q., & Van der Meeren, P. (2019). Maillard conjugate as an approach to improve whey proteins functionality: A review of conventional and novel preparation techniques. Trends in Food Science and Technology, 91(June), 1–11. https://doi.org/10.1016/j.tifs.2019.06.011
Setiowati, A. D., Rwigamba, A., & Van der Meeren, P. (2019). The influence of degree of methoxylation on the emulsifying and heat stabilizing activity of whey protein-pectin conjugates. Food Hydrocolloids, 96(September 2018), 54–64. https://doi.org/10.1016/j.foodhyd.2019.05.012
Setiowati, A. D., Saeedi, S., Wijaya, W., & Van der Meeren, P. (2017). Improved heat stability of whey protein isolate stabilized emulsions via dry heat treatment of WPI and low methoxyl pectin: Effect of pectin concentration, pH, and ionic strength. Food Hydrocolloids, 63, 716–726. https://doi.org/10.1016/j.foodhyd.2016.10.025
Setiowati, A. D., Vermeir, L., Martins, J., De Meulenaer, B., & Van der Meeren, P. (2016). Improved heat stability of protein solutions and O/W emulsions upon dry heat treatment of whey protein isolate in the presence of low-methoxyl pectin. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 510, 93–103. https://doi.org/10.1016/j.colsurfa.2016.05.034
Shang, J., Zhong, F., Zhu, S., Wang, J., & Huang, D. (2020). Structure and physiochemical characteristics of whey protein isolate conjugated with xylose through Maillard reaction at different degrees. Arabian Journal of Chemistry, 13(11), 8051–8059. https://doi.org/10.1016/j.arabjc.2020.09.034
Song, H., Chung, H., & Nam, K. (2021). Response surface modeling with Box-Behnken design for strontium removal from soil by calcium-based solution . Environmental Pollution, 274, 116577. https://doi.org/10.1016/j.envpol.2021.116577
Wang, W. D., Li, C., Bin, Z., Huang, Q., You, L. J., Chen, C., Fu, X., & Liu, R. H. (2020). Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction. International Journal of Biological Macromolecules, 150, 326–335. https://doi.org/10.1016/j.ijbiomac.2020.02.086
Xue, F., Li, C., Zhu, X., Wang, L., & Pan, S. (2013). Comparative studies on the physicochemical properties of soy protein isolate-maltodextrin and soy protein isolate-gum acacia conjugate prepared through Maillard reaction. FRIN, 51(2), 490–495. https://doi.org/10.1016/j.foodres.2013.01.012 DOI: https://doi.org/10.22146/agritech.71301
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agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.